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cd27 pe cy7 vio770  (Miltenyi Biotec)


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    Miltenyi Biotec cd27 pe cy7 vio770
    ( a ). Gating strategy of B cell subpopulations. Lymphocytes were identified by FSC (forward scatter) and SSC (side scatter). From lymphocyte population, CD19 + cells were selected, and based on them and according to IgD and <t>CD27</t> markers, naïve (IgD + CD27 - ), unswitched memory (IgD + CD27 + ), and switched memory (IgD - CD27 + ) B cells were identified. Furthermore, based on CD19 + cells and according to IgD and CD38 markers, Bm1 (IgD + CD38 - ), Bm2 (IgD + CD38 + ), Bm2’ (IgD + CD38 high ), Bm3Bm4 (IgD - CD38 high ), eBm5 (IgD - CD38 + ), and Bm5 (IgD - CD38 - ) cells were identified. Transitional type 2 cells were selected using a different gating strategy in order to obtain a pure population. For this gating strategy, CD19 + lymphocytes were selected, and from them those cells CD10 + , CD20 + , CD21 + , CD5 - , CD24 + , and CD38 + . ( b ). Gating strategy of CD4 + and CD8 + T cell subpopulations. Lymphocytes were identified by FSC (forward scatter) and SSC (side scatter). From lymphocyte population, CD3 + cells were selected, and based on them, CD4 + and CD8 + cells. Since CD4 + and CD8 + cells, and according to CD62L and CD45RO markers, naïve (CD62L + CD45RO - ), central memory (CD62L + CD45RO + ), effector memory (CD62L - CD45RO + ), and TEMRA (CD62L - CD45RO - ) T cells were identified.
    Cd27 Pe Cy7 Vio770, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd27 pe cy7 vio770/product/Miltenyi Biotec
    Average 97 stars, based on 20 article reviews
    cd27 pe cy7 vio770 - by Bioz Stars, 2026-03
    97/100 stars

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    1) Product Images from "High Pretransplant BAFF Levels and B-cell Subset Polarized towards a Memory Phenotype as Predictive Biomarkers for Antibody-Mediated Rejection"

    Article Title: High Pretransplant BAFF Levels and B-cell Subset Polarized towards a Memory Phenotype as Predictive Biomarkers for Antibody-Mediated Rejection

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms21030779

    ( a ). Gating strategy of B cell subpopulations. Lymphocytes were identified by FSC (forward scatter) and SSC (side scatter). From lymphocyte population, CD19 + cells were selected, and based on them and according to IgD and CD27 markers, naïve (IgD + CD27 - ), unswitched memory (IgD + CD27 + ), and switched memory (IgD - CD27 + ) B cells were identified. Furthermore, based on CD19 + cells and according to IgD and CD38 markers, Bm1 (IgD + CD38 - ), Bm2 (IgD + CD38 + ), Bm2’ (IgD + CD38 high ), Bm3Bm4 (IgD - CD38 high ), eBm5 (IgD - CD38 + ), and Bm5 (IgD - CD38 - ) cells were identified. Transitional type 2 cells were selected using a different gating strategy in order to obtain a pure population. For this gating strategy, CD19 + lymphocytes were selected, and from them those cells CD10 + , CD20 + , CD21 + , CD5 - , CD24 + , and CD38 + . ( b ). Gating strategy of CD4 + and CD8 + T cell subpopulations. Lymphocytes were identified by FSC (forward scatter) and SSC (side scatter). From lymphocyte population, CD3 + cells were selected, and based on them, CD4 + and CD8 + cells. Since CD4 + and CD8 + cells, and according to CD62L and CD45RO markers, naïve (CD62L + CD45RO - ), central memory (CD62L + CD45RO + ), effector memory (CD62L - CD45RO + ), and TEMRA (CD62L - CD45RO - ) T cells were identified.
    Figure Legend Snippet: ( a ). Gating strategy of B cell subpopulations. Lymphocytes were identified by FSC (forward scatter) and SSC (side scatter). From lymphocyte population, CD19 + cells were selected, and based on them and according to IgD and CD27 markers, naïve (IgD + CD27 - ), unswitched memory (IgD + CD27 + ), and switched memory (IgD - CD27 + ) B cells were identified. Furthermore, based on CD19 + cells and according to IgD and CD38 markers, Bm1 (IgD + CD38 - ), Bm2 (IgD + CD38 + ), Bm2’ (IgD + CD38 high ), Bm3Bm4 (IgD - CD38 high ), eBm5 (IgD - CD38 + ), and Bm5 (IgD - CD38 - ) cells were identified. Transitional type 2 cells were selected using a different gating strategy in order to obtain a pure population. For this gating strategy, CD19 + lymphocytes were selected, and from them those cells CD10 + , CD20 + , CD21 + , CD5 - , CD24 + , and CD38 + . ( b ). Gating strategy of CD4 + and CD8 + T cell subpopulations. Lymphocytes were identified by FSC (forward scatter) and SSC (side scatter). From lymphocyte population, CD3 + cells were selected, and based on them, CD4 + and CD8 + cells. Since CD4 + and CD8 + cells, and according to CD62L and CD45RO markers, naïve (CD62L + CD45RO - ), central memory (CD62L + CD45RO + ), effector memory (CD62L - CD45RO + ), and TEMRA (CD62L - CD45RO - ) T cells were identified.

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    cd27 pe cy7 vio770  (Miltenyi Biotec)
    97
    Miltenyi Biotec cd27 pe cy7 vio770
    ( a ). Gating strategy of B cell subpopulations. Lymphocytes were identified by FSC (forward scatter) and SSC (side scatter). From lymphocyte population, CD19 + cells were selected, and based on them and according to IgD and <t>CD27</t> markers, naïve (IgD + CD27 - ), unswitched memory (IgD + CD27 + ), and switched memory (IgD - CD27 + ) B cells were identified. Furthermore, based on CD19 + cells and according to IgD and CD38 markers, Bm1 (IgD + CD38 - ), Bm2 (IgD + CD38 + ), Bm2’ (IgD + CD38 high ), Bm3Bm4 (IgD - CD38 high ), eBm5 (IgD - CD38 + ), and Bm5 (IgD - CD38 - ) cells were identified. Transitional type 2 cells were selected using a different gating strategy in order to obtain a pure population. For this gating strategy, CD19 + lymphocytes were selected, and from them those cells CD10 + , CD20 + , CD21 + , CD5 - , CD24 + , and CD38 + . ( b ). Gating strategy of CD4 + and CD8 + T cell subpopulations. Lymphocytes were identified by FSC (forward scatter) and SSC (side scatter). From lymphocyte population, CD3 + cells were selected, and based on them, CD4 + and CD8 + cells. Since CD4 + and CD8 + cells, and according to CD62L and CD45RO markers, naïve (CD62L + CD45RO - ), central memory (CD62L + CD45RO + ), effector memory (CD62L - CD45RO + ), and TEMRA (CD62L - CD45RO - ) T cells were identified.
    Cd27 Pe Cy7 Vio770, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd27 pe cy7 vio770/product/Miltenyi Biotec
    Average 97 stars, based on 1 article reviews
    cd27 pe cy7 vio770 - by Bioz Stars, 2026-03
    97/100 stars
    		  Buy from Supplier

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    ( a ). Gating strategy of B cell subpopulations. Lymphocytes were identified by FSC (forward scatter) and SSC (side scatter). From lymphocyte population, CD19 + cells were selected, and based on them and according to IgD and CD27 markers, naïve (IgD + CD27 - ), unswitched memory (IgD + CD27 + ), and switched memory (IgD - CD27 + ) B cells were identified. Furthermore, based on CD19 + cells and according to IgD and CD38 markers, Bm1 (IgD + CD38 - ), Bm2 (IgD + CD38 + ), Bm2’ (IgD + CD38 high ), Bm3Bm4 (IgD - CD38 high ), eBm5 (IgD - CD38 + ), and Bm5 (IgD - CD38 - ) cells were identified. Transitional type 2 cells were selected using a different gating strategy in order to obtain a pure population. For this gating strategy, CD19 + lymphocytes were selected, and from them those cells CD10 + , CD20 + , CD21 + , CD5 - , CD24 + , and CD38 + . ( b ). Gating strategy of CD4 + and CD8 + T cell subpopulations. Lymphocytes were identified by FSC (forward scatter) and SSC (side scatter). From lymphocyte population, CD3 + cells were selected, and based on them, CD4 + and CD8 + cells. Since CD4 + and CD8 + cells, and according to CD62L and CD45RO markers, naïve (CD62L + CD45RO - ), central memory (CD62L + CD45RO + ), effector memory (CD62L - CD45RO + ), and TEMRA (CD62L - CD45RO - ) T cells were identified.

    Journal: International Journal of Molecular Sciences

    Article Title: High Pretransplant BAFF Levels and B-cell Subset Polarized towards a Memory Phenotype as Predictive Biomarkers for Antibody-Mediated Rejection

    doi: 10.3390/ijms21030779

    Figure Lengend Snippet: ( a ). Gating strategy of B cell subpopulations. Lymphocytes were identified by FSC (forward scatter) and SSC (side scatter). From lymphocyte population, CD19 + cells were selected, and based on them and according to IgD and CD27 markers, naïve (IgD + CD27 - ), unswitched memory (IgD + CD27 + ), and switched memory (IgD - CD27 + ) B cells were identified. Furthermore, based on CD19 + cells and according to IgD and CD38 markers, Bm1 (IgD + CD38 - ), Bm2 (IgD + CD38 + ), Bm2’ (IgD + CD38 high ), Bm3Bm4 (IgD - CD38 high ), eBm5 (IgD - CD38 + ), and Bm5 (IgD - CD38 - ) cells were identified. Transitional type 2 cells were selected using a different gating strategy in order to obtain a pure population. For this gating strategy, CD19 + lymphocytes were selected, and from them those cells CD10 + , CD20 + , CD21 + , CD5 - , CD24 + , and CD38 + . ( b ). Gating strategy of CD4 + and CD8 + T cell subpopulations. Lymphocytes were identified by FSC (forward scatter) and SSC (side scatter). From lymphocyte population, CD3 + cells were selected, and based on them, CD4 + and CD8 + cells. Since CD4 + and CD8 + cells, and according to CD62L and CD45RO markers, naïve (CD62L + CD45RO - ), central memory (CD62L + CD45RO + ), effector memory (CD62L - CD45RO + ), and TEMRA (CD62L - CD45RO - ) T cells were identified.

    Article Snippet: The following monoclonal antibodies were used to T cell subpopulations identification: anti-CD62L-FITC clone DREG56 (Beckman Coulter, Brea, CA, USA), CD45RO-PE clone UCHL1 (BD Biosciences, San Diego, CA), CD28-PC5.5 clone L293, CD27-PE Cy7 Vio770 clone 1A4CD27, CCR7-APC clone REA108 (Miltenyi Biotec, Bergisch Gladbach, Germany), CD4-APC Vio770 clone VIT4, and CD3-VioBlue clone UCHT1 (Immunostep, Salamanca, Spain).

    Techniques: